Elucidating genetic relationships, diversity and population structure among the Turkish female figs

Yazan: admin Tarih: Oca 21st, 2010 | Kategori:: Gene polymorphisms

Ikten H (Ikten, Hatice)2, Mutlu N (Mutlu, Nedim)3, Gulsen O (Gulsen, Osman)1, Kocatas H (Kocatas, Hilmi)4, Aksoy U (Aksoy, Uygun)5

Source: GENETICA Volume: 138 Issue: 2 Pages: 169-177 Published: FEB 2010

Abstract: A collection of 96 female Turkish fig (Ficus carica L.) accessions was studied to elucidate genetic structure and estimate diversity and genetic similarity distribution among the female figs present in Turkish genetic resources, using 157 molecular genome markers including 129 sequence-related amplified polymorphisms, 21 random amplified polymorphic DNAs, and 7 simple-sequence repeats. The plant samples mainly included Turkish fig collections selected throughout the country over the course of a half-century. Neighbor-joining analysis revealed continuous dissimilarity range, and it was difficult to classify figs into distinct groups. The principle component analysis produced similar results. The analysis of molecular variance indicated that 95 and 93% of genetic variation were explained by within geographic origins and similar fruit rind color, respectively. Sub-structuring Bayesian analysis assigned the 96 female figs into four sub-populations, and indicated that they were highly related. The corrected allelic pairwise distances among the six geographic origins were less than 5%. This study suggests that geography- and color-based groups were not genetically distinct among the Turkish figs.
Document Type: Article
Language: English
Author Keywords: Ficus carica; SRAP; Neighbor-joining; PCA; AMOVA; Population structure
KeyWords Plus: FICUS-CARICA L.; GERMPLASM COLLECTION; COMMON FIG; MARKERS; RAPD; RELATEDNESS; GENOTYPES; RFLP; AFLP
Reprint Address: Gulsen, O (reprint author), Erciyes Univ, Dept Hort, Fac Agr, TR-38039 Kayseri, Turkey
Addresses:
1. Erciyes Univ, Dept Hort, Fac Agr, TR-38039 Kayseri, Turkey
2. Minist Agr & Rural Affairs, W Mediterranean Res Inst, TR-07100 Antalya, Turkey
3. Univ Nebraska, George W Beadle Ctr, Dept Biochem, Lincoln, NE 68503 USA
4. Fig Res Inst, TR-09600 Erbeyli, Aydin Turkey
5. Aegean Univ, Fac Agr, Dept Hort, TR-35100 Izmir, Turkey
E-mail Addresses: o_gulsen@yahoo.com


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Common MEFV mutations and polymorphisms in an elderly population: an association with E148Q polymorphism andrheumatoid factor levels.

Yazan: admin Tarih: Ağu 24th, 2009 | Kategori:: Cancer (Kanser)

Clin Exp Rheumatol. 2009 Mar-Apr;27(2):340-3. Links

Turanli ET, Beger T, Erdincler D, Curgunlu A, Karaman S, Karaca E, Dasdemir S, Bolayirli M, Yazici H.

Department of Molecular Biology and Genetics, and 2Molecular Biology and Biotechnology Research Center, Istanbul Technical University, Istanbul, Turkey. turanlie@itu.edu.tr

OBJECTIVES: To analyse the most common MEFV (Mediterranean fever gene) mutations and polymorphisms in an elderly population free of chronic inflammatory disease (n=164), and explore possible associations between hsCRP (high sensitive C-reactive protein) and RF (rheumatoid factor) levels with MEFV mutations and polymorphisms. METHODS: An elderly group free of chronic inflammatory disease was chosen among the outpatients of the division of geriatric medicine. Total genomic DNA was isolated from blood, and PCR-RFLP analysis was performed using established protocols. Sera were analyzed for hsCRP and RF levels. RESULTS: The frequencies for 694V (1.8%), 694I (1.8%), 680I (0.6%), 726A (2.1%) and 148Q (5%) alleles were found to be similar to Turkish historic controls, with a carrier frequency of 1/4. Further analyses with rheumatoid factor (RF) levels and mutations revealed a significant association between the presence of the E148Q polymorphism with increased RF levels (>15 mg/dl) (xi2= 7.358, p=0.007, OR=5.41 95% CI 1.41-20.64). CONCLUSIONS: Common MEFV mutations and polymorphisms were similarly represented among the elderly population compared to historic controls. On the other hand, a significant association was found between the presence of E148Q polymorphism and increased RF levels. This suggests that the previously noted increased RF levels in elderly populations may somehow be related to the now described association of RF with MEFV E148Q polymorphism.


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Common MEFV mutations and polymorphisms in an elderly population: an association with E148Q polymorphism andrheumatoid factor levels.

Yazan: admin Tarih: Tem 12th, 2009 | Kategori:: Gene polymorphisms, polymorphisms

Clin Exp Rheumatol. 2009 Mar-Apr;27(2):340-3. Links

Turanli ET, Beger T, Erdincler D, Curgunlu A, Karaman S, Karaca E, Dasdemir S, Bolayirli M, Yazici H.Department of Molecular Biology and Genetics, and 2Molecular Biology and Biotechnology Research Center, Istanbul Technical University, Istanbul, Turkey. turanlie@itu.edu.tr

OBJECTIVES: To analyse the most common MEFV (Mediterranean fever gene) mutations and polymorphisms in an elderly population free of chronic inflammatory disease (n=164), and explore possible associations between hsCRP (high sensitive C-reactive protein) and RF (rheumatoid factor) levels with MEFV mutations and polymorphisms. METHODS: An elderly group free of chronic inflammatory disease was chosen among the outpatients of the division of geriatric medicine. Total genomic DNA was isolated from blood, and PCR-RFLP analysis was performed using established protocols. Sera were analyzed for hsCRP and RF levels. RESULTS: The frequencies for 694V (1.8%), 694I (1.8%), 680I (0.6%), 726A (2.1%) and 148Q (5%) alleles were found to be similar to Turkish historic controls, with a carrier frequency of 1/4. Further analyses with rheumatoid factor (RF) levels and mutations revealed a significant association between the presence of the E148Q polymorphism with increased RF levels (>15 mg/dl) (xi2= 7.358, p=0.007, OR=5.41 95% CI 1.41-20.64). CONCLUSIONS: Common MEFV mutations and polymorphisms were similarly represented among the elderly population compared to historic controls. On the other hand, a significant association was found between the presence of E148Q polymorphism and increased RF levels. This suggests that the previously noted increased RF levels in elderly populations may somehow be related to the now described association of RF with MEFV E148Q polymorphism.


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