Genetic polymorphisms of sulfotransferases (SULT1A1 and SULT1A2) in a Turkish population.

Yazan: admin Tarih: Oca 25th, 2011 | Kategori:: SULT1A1, SULT1A2, Sulfotransferases

Biochem Genet. 2010 Dec;48(11-12):987-94. Epub 2010 Oct 10.

Arslan S.

Department of Molecular Biology and Genetics, Cumhuriyet University, Sivas, Turkey. arserdal@yahoo.com

Abstract

Sulfotransferases (SULTs) play a significant role in the biotransformation of a variety of xenobiotics and endogenous compounds. SULTs are genetically polymorphic enzymes; to date, 12 human cytosolic SULT isoforms have been identified. This study investigated SULT1A1 and SULT1A2 gene polymorphism using a PCR-RFLP method (n = 303). The frequency of the SULT1A1*1 allele was 76.2% and SULT1A1*2 was 23.8%. The SULT1A1*3 allele could not be identified. The SULT1A2 frequencies were 69.2% (SULT1A2*1), 18.3% (SULT1A2*2), and 12.5% (SULT1A2*3). The SULT1A1 and SULT1A2 loci were in Hardy-Weinberg equilibrium (SULT1A1 χ² = 0.58, P = 0.44; SULT1A2 χ² = 7.28, P = 0.06). Linkage analysis indicated a close linkage between these two genes (χ² = 5.31, P < 0.01); therefore, the statistical hypothesis that SULT1A1 and SULT1A2 alleles are independently distributed was rejected. Additionally, a strongly positive linkage was detected between SULT1A1*2 and SULT1A2*2 alleles in this population (D’ = 0.79, χ² = 33.33).


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Genetic Polymorphisms of Sulfotransferases (SULT1A1 and SULT1A2) in a Turkish Population.

Yazan: admin Tarih: Kas 30th, 2010 | Kategori:: SULT1A1, SULT1A2, Sulfotransferases

Biochem Genet. 2010 Dec;48(11-12):987-94. Arslan S.

Department of Molecular Biology and Genetics, Faculty of Science and Literature, Cumhuriyet University, 58140, Sivas, Turkey, arserdal@yahoo.com.

Abstract

Sulfotransferases (SULTs) play a significant role in the biotransformation of a variety of xenobiotics and endogenous compounds. SULTs are genetically polymorphic enzymes; to date, 12 human cytosolic SULT isoforms have been identified. This study investigated SULT1A1 and SULT1A2 gene polymorphism using a PCR-RFLP method (n = 303). The frequency of the SULT1A1*1 allele was 76.2% and SULT1A1*2 was 23.8%. The SULT1A1*3 allele could not be identified. The SULT1A2 frequencies were 69.2% (SULT1A2*1), 18.3% (SULT1A2*2), and 12.5% (SULT1A2*3). The SULT1A1 and SULT1A2 loci were in Hardy-Weinberg equilibrium (SULT1A1 χ(2) = 0.58, P = 0.44; SULT1A2 χ(2) = 7.28, P = 0.06). Linkage analysis indicated a close linkage between these two genes (χ(2) = 5.31, P < 0.01); therefore, the statistical hypothesis that SULT1A1 and SULT1A2 alleles are independently distributed was rejected. Additionally, a strongly positive linkage was detected between SULT1A1*2 and SULT1A2*2 alleles in this population (D’ = 0.79, χ(2) = 33.33).


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I405V and TaqIB polymorphisms of the cholesteryl ester transfer protein and their relation to serum lipid and lipoprotein levels in a Turkish population

Yazan: admin Tarih: Nis 22nd, 2009 | Kategori:: Kategorilenmemiş

Semra Doru-Abbasolu 1 *, Hande Parldar-Karpuzolu 1, Bilge Depboylu 1, Naci Çine 2, Müjdat Uysal 1, Gülçin Aykaç-Toker 1
1Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
2Department of Medical Genetics, Kocaeli University Faculty of Medicine, Kocaeli, Turkey
 
email: Semra Doru-Abbasolu (sdabbasoglu@yahoo.com)

*Correspondence to Semra Doru-Abbasolu, Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Çapa 34093, Istanbul, Turkey.

Keywords
cholesteryl ester transfer protein (CETP) • genetic polymorphism • TaqIB • I405V
Abstract
Cholesteryl ester transfer protein (CETP) plays a central role in high-density lipoprotein (HDL) metabolism. Genetic polymorphisms of the CETP gene can influence levels of serum lipoproteins. It has been reported that mean HDL-cholesterol (HDL-C) concentrations are low in Turkish population. Thus, we investigated the frequencies of the common I405V and TaqIB polymorphisms of the CETP gene and their relation to serum lipid and lipoprotein levels in a Turkish population. The variant allele frequencies of I405V and TaqIB polymorphisms of the CETP gene were found to be 0.38 and 0.46, respectively and similar to some of the European populations. Subjects for the VV genotype of I405V polymorphism had higher HDL-C levels than did II subjects. The covariance analysis showed that gender and triglyceride (TG) levels have an effect on the association of HDL-C and I405V polymorphism. In conclusion, our results indicate that I405V polymorphism may affect the HDL-C levels in Turkish population. The association of this polymorphism and HDL-C levels could be modified by other factors, such as gender and TG levels. Copyright © 2009 John Wiley & Sons, Ltd.

 

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Received: 6 November 2008; Accepted: 17 November 2008
Digital Object Identifier (DOI)

10.1002/cbf.1536  About DOI


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Comparison of IL10 and IL2 genotypes of Turkish population with other populations.

Yazan: admin Tarih: Nis 11th, 2009 | Kategori:: Gene polymorphisms

Int J Immunogenet. 2009 Apr;36(2):97-101.

Comparison of IL10 and IL2 genotypes of Turkish population with other populations.

Yazici AC, Atac FB, Verdi H, Ozbek N.

Department of Biostatistics, Başkent University, Ankara, Turkey.

The human genome has been shaped by evolutionary and historical forces. Therefore, genetic polymorphisms are useful tools not only to understand the susceptibility to disease in modern populations, but the history of ancestral populations as well. For this purpose, data on genetic polymorphisms such as human leucocyte antigen, mitochondrial DNA sequence variability and the frequencies of TAP1 and TAP2 gene variants in Turkey have been reported previously. Here we have used interleukin (IL)-10 (-592C/A, -819T/C, -1082G/A) and IL-2 (-330T/G) as genetic markers to study the relationship between Turkish population and other populations.


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